黑色素瘤中microRNA的基因表达差异_科研在线_抗癌健康网 - 开户送体验金
欢迎来到抗癌健康网!  
放入收藏夹 | 返回首页

开户送体验金

2019-01-30

抗癌健康网

手机看网页

导读:恶性黑色素瘤的发病率正在逐年上升,并且其上升速度比任何其他癌症都要快。

恶性黑色素瘤的发病率正在逐年上升,并且其上升速度比任何其他癌症都要快。皮肤切除活检仍然是黑色素瘤诊断和预后判断的标准方法。良性和恶性病变组织之间有显著的形态重叠,肿瘤的厚度并不总是一个准确的预测指标。

因此确定新的分子标记物以支持组织学检查非常有必要,近日研究人员对不同病理阶段的福尔马林固定、石蜡包埋的样本进行微陈列分析,以便识别差异表达的微小RNA(miRNA)。相关研究论文发表在British Journal of Cancer杂志上。

采用QRT-PCR验证这些微小RNA的差异表达,miRNA的功能学研究采用黑色素瘤细胞转染能调节miRNA表达的miRNA前体或抑制剂来开展。结果发现有20个miRNA在良性痣和原发性或转移性黑色瘤中存在显著差异性表达,而其中大部分在黑色素瘤中是下调的,而只有2个miRNA即miR-203和miR-205在原发性和转移性黑色素瘤中是差异表达的。

在体外试验中,抑制过度的miR-200C和miR-205能显著抑制黑色素瘤细胞的克隆集落形成能力,抑制过表达miR-211既能集落形成能力,也能抑制黑色素瘤细胞的侵袭。该研究所确定的一系列miRNA差异表达可能用于黑色素瘤的诊断或预后指标,其中3个miRNAs(即miR-200c、miR-205和miR-211)发挥肿瘤抑制基因的功能。(生物谷:Bioon.com)

doi:10.1038/bjc.2011.568
PMC:
PMID:

Differential expression of microRNAs during melanoma progression: miR-200c, miR-205 and miR-211 are downregulated in melanoma and act as tumour suppressors.

Xu Y, Brenn T, Brown ER, Doherty V, Melton DW.

BACKGROUND:

The incidence of malignant melanoma is increasing faster than that for any other cancer. Histological examination of skin excision biopsies remains the standard method for melanoma diagnosis and prognosis. Significant morphological overlap between benign and malignant lesions complicates diagnosis, and tumour thickness is not always an accurate predictor of prognosis.

METHODS:

To identify improved molecular markers to support histological examination, we used microarray analysis of formalin-fixed and paraffin-embedded samples from different stages of melanomagenesis to identify differentially expressed microRNAs (miRNAs). Differential expression was validated by qRT-PCR, and functional studies were carried out after transfection of miRNA precursors or inhibitors into melanoma cells to modulate miRNA expression.

RESULTS:

In all, 20 miRNAs showed highly significant differential expression between benign naevi and either primary or metastatic melanomas, the majority being downregulated in melanoma, whereas only 2 miRNAs, namely miR-203 and miR-205, were differentially expressed between primary and metastatic melanomas. In functional in vitro assays, overexpression of miR-200c and miR-205 inhibited anchorage-independent colony formation and overexpression of miR-211 inhibited both anchorage-independent colony formation and invasion.

CONCLUSION:

We have identified a series of differentially expressed miRNAs that could be useful as diagnostic or prognostic markers for melanoma and have shown that three miRNAs (namely miR-200c, miR-205 and miR-211) act as tumour suppressors.

在这里,掌握健康资讯

扫描左侧二维码即可添加抗癌健康网官方微信;
健康之路 与你同行
胆囊癌更多>>
尚有肉瘤、类癌、原发性恶性黑色素瘤、巨细胞腺癌等。原发性胆囊癌临床上较为少见。[详情]
热文欣赏更多>>

健康阅读

推荐欣赏

  • 热门推荐
  • 最新期刊
  • 疾病关注
长期憋尿,膀胱癌,长期憋尿引发膀胱癌
[详情]
白血病与骨髓移植术
[详情]
筷子致癌?靠什么吃饭?
[详情]
卵巢囊肿并不可怕
[详情]
调理内分泌多吃黄色2017开户送体验金
[详情]
吃腐乳致癌可信吗?
[详情]
资讯排行榜更多>>
肺癌康复指南吃什么?
信息聚焦更多>>
化疗药物中成药止痛药
卡莫司汀复方鹿仙草颗粒阿司匹林
优福定慈丹胶囊扑热息痛
替加氟西黄丸盐酸哌替啶片
奥沙利铂臌症丸吗啡
吉西他滨贞芪扶正颗粒芬太尼
视频推荐更多>>
肿瘤热文欣赏更多>>

特别声明:本站信息仅供参考 不能作为诊断及医疗的依据平台联系我们

抗癌健康网 版权所有 未经授权请勿转载  CopyRight©2008-2018 www.gaming-nexus.net, All Rights Reserved. 浙ICP备11014848号